Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.virol.2010.12.059
Titel (primär) Deletion analysis of the 3' long terminal repeat sequence of plant retrotransposon Tto1 identifies 125 base pairs redundancy as sufficient for first strand transfer
Autor Tramontano, A.; Donath, A.; Bernhart, S.H.; Reiche, K.; Böhmdorfer, C.; Stadler, P.F.; Bachmair, A.
Quelle Virology
Erscheinungsjahr 2011
Department PROTEOM
Band/Volume 412
Heft 1
Seite von 75
Seite bis 82
Sprache englisch
Keywords First strand transfer; Long terminal repeat; Redundancy region; Retrotransposon; Tto1
Abstract

Retroviruses and many retrotransposons are flanked by sequence repeats called long terminal repeats (LTRs). These sequences contain a promoter region, which is active in the 5′ LTR, and transcription termination signals, which are active in the LTR copy present at the 3′ end. A section in the middle of the LTR, called Redundancy region, occurs at both ends of the mRNA. Here we show that in the copia type retrotransposon Tto1, the promoter and terminator functions of the LTR can be supplied by heterologous sequences, thereby converting the LTR into a significantly shorter sub-terminal repeat. An engineered Tto1 element with 125 instead of the usual 574 base pairs repeated in the 5′ and 3′ region can still promote strand transfer during cDNA synthesis, defining a minimal Redundancy region for this element. Based on this finding, we propose a model for first strand transfer of Tto1.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12135
Tramontano, A., Donath, A., Bernhart, S.H., Reiche, K., Böhmdorfer, C., Stadler, P.F., Bachmair, A. (2011):
Deletion analysis of the 3' long terminal repeat sequence of plant retrotransposon Tto1 identifies 125 base pairs redundancy as sufficient for first strand transfer
Virology 412 (1), 75 - 82 10.1016/j.virol.2010.12.059