Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.3732/ajb.1100322 |
Titel (primär) | Identification and characterization of microsatellite loci in the rush Juncus effusus (Juncaceae) |
Autor | Michalski, S.G.; Durka, W. |
Quelle | American Journal of Botany |
Erscheinungsjahr | 2012 |
Department | BZF |
Band/Volume | 99 |
Heft | 2 |
Seite von | e53 |
Seite bis | e55 |
Sprache | englisch |
Keywords | 454 sequencing; inbreeding; Juncaceae; Juncus effusus; wetland |
Abstract | • Premise of the study: Microsatellite markers were developed for the common wetland plant Juncus effusus to assess levels of within- and among-population genetic diversity. • Methods and Results: Using a next-generation sequencing approach we identified new microsatellite loci, from which 23 were further characterized in a large population of J. effusus. Expected heterozygosity ranged from 0 to 0.64 with a mean of 0.407. Inbreeding coefficients (mean = 0.984) were very high, confirming earlier suggestions of an inbred mating system. Cross-amplification was tested in J. conglomeratus, J. inflexus, J. drummondii, and J. filiformis, with best results in the former. • Conclusions: The described microsatellite markers will be helpful in assisting studies on, e.g., reproduction, taxonomy, and phylogeography in J. effusus and related species. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=12130 |
Michalski, S.G., Durka, W. (2012): Identification and characterization of microsatellite loci in the rush Juncus effusus (Juncaceae) Am. J. Bot. 99 (2), e53 - e55 10.3732/ajb.1100322 |