Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1128/AEM.01812-07
Titel (primär) Novel combination of atomic force microscopy and epifluorescence microscopy for visualization of leaching bacteria on pyrite
Autor Mangold, S.; Harneit, K.; Rohwerder, T.; Claus, G.; Sand, W.
Quelle Applied and Environmental Microbiology
Erscheinungsjahr 2008
Department UMB
Band/Volume 74
Heft 2
Seite von 410
Seite bis 415
Sprache englisch
Abstract

Bioleaching of metal sulfides is an interfacial process comprising the interactions of attached bacterial cells and bacterial extracellular polymeric substances with the surface of a mineral sulfide. Such processes and the associated biofilms can be investigated at high spatial resolution using atomic force microscopy (AFM). Therefore, we visualized biofilms of the meso-acidophilic leaching bacterium Acidithiobacillus ferrooxidans strain A2 on the metal sulfide pyrite with a newly developed combination of AFM with epifluorescence microscopy (EFM). This novel system allowed the imaging of the same sample location with both instruments. The pyrite sample, as fixed on a shuttle stage, was transferred between AFM and EFM devices. By staining the bacterial DNA with a specific fluorescence dye, bacterial cells were labeled and could easily be distinguished from other topographic features occurring in the AFM image. AFM scanning in liquid caused deformation and detachment of cells, but scanning in air had no effect on cell integrity. In summary, we successfully demonstrate that the new microscopic system was applicable for visualizing bioleaching samples. Moreover, the combination of AFM and EFM in general seems to be a powerful tool for investigations of biofilms on opaque materials and will help to advance our knowledge of biological interfacial processes. In principle, the shuttle stage can be transferred to additional instruments, and combinations of AFM and EFM with other surface-analyzing devices can be proposed.

dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1206
Mangold, S., Harneit, K., Rohwerder, T., Claus, G., Sand, W. (2008):
Novel combination of atomic force microscopy and epifluorescence microscopy for visualization of leaching bacteria on pyrite
Appl. Environ. Microb. 74 (2), 410 - 415 10.1128/AEM.01812-07