Dr. Christian Eberlein
Contact / Address:
Phone: +49 341 235 1765
Office: building 1.0, room 114
Curriculum Vitae
| 2015 - present | Postdoc researcher, Helmholtz Centre for Environmental Research - UFZ, Department of Molecular Environmental Biotechnology |
| 2013 - 2015 | Postdoc researcher at the University Hospital Carl Gustav Carus |
| 2009 - 2013 | PhD in Biochemistry/Microbiology, University of Leipzig and University of Freiburg, within EU-Project MAGICPAH |
| 2009 | Diploma in Biology, University of Leipzig |
Research
I am part of the project "Use and management of finest particulate anthropogenic material flows in a sustainable circular economy – FINEST" (2022-2027) funded by the Helmholtz Association (Helmholtz Sustainability Challenge). FINEST
My research is also embedded in the research project NO-STRESS, which is funded by the Federal Ministry of Education and Research of Germany (Bundesministerium für Bildung und Forschung, BMBF). Together with the Helmholtz Centre Jülich and the University of Düsseldorf we want to provide robust bacterial cell factories with high tolerance to key chemical stressors. Such cell factories can be used for the biotechnological production of fine chemicals used as pharmaceuticals or chemical building blocks. NO-STRESS
I was part of the EU-Project P4SB (From Plastic waste to Plastic value using Pseudomonas putida Synthetic Biology; P4SB; 2015-2019) which aimed to transform conventional plastics gained from fossil fuels into bioplastics; employing Pseudomonas putida for the degradation of oligomeric resins.
Publications
2024 (3)
- Bitzenhofer, N.L., Höfel, C., Thies, S., Weiler, A.J., Eberlein, C., Heipieper, H.J., Batra-Safferling, R., Sundermeyer, P., Heidler, T., Sachse, C., Busche, T., Kalinowski, J., Belthle, T., Drepper, T., Jaeger, K.-E., Loeschcke, A. (2024):
Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis
Microb. Biotechnol. 17 (1), e14312 10.1111/1751-7915.14312 - Lisiecka, N., Parus, A., Simpson, M., Kloziński, A., Zembrzuska, J., Frankowski, R., Zgoła-Grześkowiak, A., Woźniak-Karczewska, M., Siwińska-Ciesielczyk, K., Niemczak, M., Sandomierski, M., Eberlein, C., Heipieper, H.J., Chrzanowski, Ł. (2024):
Unraveling the effects of acrylonitrile butadiene styrene (ABS) microplastic ageing on the sorption and toxicity of ionic liquids with 2,4-D and glyphosate herbicides
Chemosphere 364 , art. 143271 10.1016/j.chemosphere.2024.143271 - Strotmann, U., Durand, M.-J., Thouand, G., Eberlein, C., Heipieper, H.J., Gartiser, S., Pagga, U. (2024):
Microbiological toxicity tests using standardized ISO/OECD methods-current state and outlook
Appl. Microbiol. Biotechnol. 108 , art. 454 10.1007/s00253-024-13286-0
2023 (3)
- Cárdenas Espinosa, M.J., Schmidgall, T., Pohl, J., Wagner, G., Wynands, B., Wierckx, N., Heipieper, H.J., Eberlein, C. (2023):
Assessment of new and genome-reduced Pseudomonas strains regarding their robustness as chassis in biotechnological applications
Microorganisms 11 (4), art. 837 10.3390/microorganisms11040837 - Schwanemann, T., Urban, E.A., Eberlein, C., Gätgens, J., Rago, D., Krink, M., Nikel, P.I., Heipieper, H.J., Wynands, B., Wierckx, N. (2023):
Production of (hydroxy)benzoate-derived polyketides by engineered Pseudomonas with in situ extraction
Bioresour. Technol. 388 , art. 129741 10.1016/j.biortech.2023.129741 - Wynands, B., Wierckx, N., Heipieper, H.J., Eberlein, C. (2023):
Pseudomonas taiwanensis VLB120 als Plattform für die Biotechnologie
Biospektrum 29 (6), 686 - 688 10.1007/s12268-023-2015-7
2022 (2)
- Puiggené, Ò., Cárdenas Espinosa, M.J., Schlosser, D., Thies, S., Jehmlich, N., Kappelmeyer, U., Schreiber, S., Wibberg, D., Kalinowski, J., Harms, H., Heipieper, H.J., Eberlein, C. (2022):
Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1
Sci. Rep. 12 , art. 2666 10.1038/s41598-022-06558-0 - Riesbeck, S., Petruschke, H., Rolle-Kampczyk, U., Schori, C., Ahrens, C.H., Eberlein, C., Heipieper, H.J., von Bergen, M., Jehmlich, N. (2022):
Adaptation and resistance: How Bacteroides thetaiotaomicron copes with the bisphenol A substitute bisphenol F
Microorganisms 10 (8), art. 1610 10.3390/microorganisms10081610
2021 (4)
- Bitzenhofer, N.L., Kruse, L., Thies, S., Wynands, B., Lechtenberg, T., Rönitz, J., Kozaeva, E., Wirth, N.T., Eberlein, C., Jaeger, K.-E., Nikel, P.I., Heipieper, H.J., Wierckx, N., Loeschcke, A. (2021):
Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways
Essays Biochem. 65 (2), 319 - 336 10.1042/EBC20200173 - Cárdenas Espinosa, M.J., Colina Blanco, A., Heipieper, H.J., Eberlein, C. (2021):
Screening and cultivating microbial strains able to grow on building blocks of polyurethane
In: Weber, G., Bornscheuer, U.T., Wei, R. (eds.)
Enzymatic plastic degradation
Methods Enzymol. 648
Elsevier, p. 423 - 434 10.1016/bs.mie.2020.12.008 - Cárdenas Espinosa, M.J., Schmidgall, T., Wagner, G., Kappelmeyer, U., Schreiber, S., Heipieper, H.J., Eberlein, C. (2021):
An optimized method for RNA extraction from the polyurethane oligomer degrading strain Pseudomonas capeferrum TDA1 growing on aromatic substrates such as phenol and 2,4-diaminotoluene
PLOS One 16 (11), e0260002 10.1371/journal.pone.0260002 - Utomo, R.N.C., Heipieper, H.J., Eberlein, C., Blank, L.M. (2021):
New species Pseudomonas capeferrum TDA1 as a plastic monomer utilizer and a PHA native producer
IOP Conf. Ser.: Earth Environ. Sci. 894 (1), art. 012024 10.1088/1755-1315/894/1/012024
2020 (5)
- Blesken, C.C., Bator, I., Eberlein, C., Heipieper, H.J., Tiso, T., Blank, L.M. (2020):
Genetic cell-surface modification for optimized foam fractionation
Front. Bioeng. Biotechnol. 8 , art. 572892 10.3389/fbioe.2020.572892 - Cárdenas Espinosa, M.J., Colina Blanco, A., Schmidgall, T., Atanasoff-Kardjalieff, A.K., Kappelmeyer, U., Tischler, D., Pieper, D.H., Heipieper, H.J., Eberlein, C. (2020):
Towards biorecycling: Isolation of a soil bacterium that grows on a polyurethane oligomer and monomer
Front. Microbiol. 11 , art. 404 10.3389/fmicb.2020.00404 - Schäpe, S.S., Krause, J.L., Masanetz, R.K., Riesbeck, S., Starke, R., Rolle-Kampczyk, U., Eberlein, C., Heipieper, H.J., Herberth, G., von Bergen, M., Jehmlich, N. (2020):
Environmentally relevant concentration of bisphenol S shows slight effects on SIHUMIx
Microorganisms 8 (9), art. 1436 10.3390/microorganisms8091436 - Tiso, T., Ballerstedt, H., Eberlein, C., Zimmermann, W., Wierckx, N., Blank, L.M. (2020):
Von Plastikmüll zu Plastikwertstoffen – Polymerrecycling neu gedacht
Biospektrum 26 (2), 212 - 214 10.1007/s12268-020-1349-7 - Utomo, R.N.C., Li, W.-J., Tiso, T., Eberlein, C., Doeker, M., Heipieper, H.J., Jupke, A., Wierckx, N., Blank, L.M. (2020):
Defined microbial mixed culture for utilization of polyurethane monomers
ACS Sustain. Chem. Eng. 8 (47), 17466 - 17474 10.1021/acssuschemeng.0c06019
2019 (2)
- Eberlein, C., Starke, S., Escobar Doncel, Á., Scarabotti, F., Heipieper, H.J. (2019):
Quantification of outer membrane vesicles: a potential tool to compare response in Pseudomonas putida KT2440 to stress caused by alkanols
Appl. Microbiol. Biotechnol. 103 (10), 4193 - 4201 10.1007/s00253-019-09812-0 - Wierckx, N., Narancic, T., Eberlein, C., Wei, R., Drzyzga, O., Magnin, A., Ballerstedt, H., Kenny, S., Pollet, E., Avérous, L., O’Connor, K.E., Zimmermann, W., Heipieper, H.J., Prieto, A., Jiménez, J., Blank, L.M. (2019):
Plastic biodegradation: challenges and opportunities
In: Steffan, R. (ed.)
Consequences of microbial interactions with hydrocarbons, oils, and lipids: Biodegradation and bioremediation
Handbook of Hydrocarbon and Lipid Microbiology
Springer International Publishing, Cham, p. 333 - 361 10.1007/978-3-319-44535-9_23-1
2018 (2)
- Eberlein, C., Baumgarten, T., Starke, S., Heipieper, H.J. (2018):
Immediate response mechanisms of Gram-negative solvent-tolerant bacteria to cope with environmental stress: cis-trans isomerization of unsaturated fatty acids and outer membrane vesicle secretion
Appl. Microbiol. Biotechnol. 102 (6), 2583 - 2593 10.1007/s00253-018-8832-9 - Heipieper, H.J., Pepi, M., Baumgarten, T., Eberlein, C. (2018):
Surface properties and cellular energetics of bacteria in response to the presence of hydrocarbons
In: Krell, T. (ed.)
Cellular ecophysiology of microbe: Hydrocarbon and lipid interactions
Handbook of Hydrocarbon and Lipid Microbiology
Springer International Publishing, Cham, p. 397 - 408 10.1007/978-3-319-50542-8_50
2010 (1)
- Löffler, C., Eberlein, C., Mäusezahl, I., Kappelmeyer, U., Heipieper, H.J. (2010):
Physiological evidence for the presence of a cis-trans isomerase of unsaturated fatty acids in Methylococcus capsulatus Bath to adapt to the presence of toxic organic compounds
FEMS Microbiol. Lett. 308 (1), 68 - 75 10.1111/j.1574-6968.2010.01993.x
Other Publications
Georgi JA, Taube F, Kramer M, Herold S, Rollig C, Eberlein C, Stasik S, Kramer A, Berdel WE, Serve H, Platzbecker U, Bornhauser M, Ehninger G, Schetelig J, Thiede C, SAL SL (2016) Mutational profiling of AML patients with CEBPA-mutations reveals differences between biallelic and monoallelic TAD and bZIP mutant subgroups. Oncology Research and Treatment 39:45-45.
Georgi JA, Taube F, Kramer M, Herold S, Schaefer-Eckart K, Schmitz N, Eberlein C, Stasik S, Aulitzky WE, Kraemer A, Roesler W, Haenel M, Einsele H, Baldus CD, Middeke JM, Platzbecker U, Roellig C, Serve H, Berdel WE, Ehninger G, Bornhaeuser M, Schetelig J, Thiede C (2016) Differences Between CEBPA bZIP and TAD Mutations and Their Effect on Outcome-an Analysis in 4578 Patients with Acute Myeloid Leukemia. Blood 128(22):283
Juarez JF, Liu H, Zamarro MT, McMahon S, Liu H, Naismith JH, Eberlein C, Boll M, Carmona M, Diaz E (2015) Unraveling the specific regulation of the central pathway for anaerobic degradation of 3-methylbenzoate. J Biol Chem 290(19):12165-1283. doi:10.1074/jbc.M115.637074
Juarez JF, Zamarro MT, Eberlein C, Boll M, Carmona M, Diaz E (2013) Characterization of the mbd cluster encoding the anaerobic 3-methylbenzoyl-CoA central pathway. Environ Microbiol 15(1):148-166. doi:10.1111/j.1462-2920.2012.02818.x
Eberlein C, Estelmann S, Seifert J, von Bergen M, Muller M, Meckenstock RU, Boll M (2013) Identification and characterization of 2-naphthoyl-coenzyme A reductase, the prototype of a novel class of dearomatizing reductases. Mol Microbiol 88(5):1032-1039. doi:10.1111/mmi.12238
Eberlein C, Johannes J, Mouttaki H, Sadeghi M, Golding BT, Boll M, Meckenstock RU (2013) ATP-dependent/-independent enzymatic ring reductions involved in the anaerobic catabolism of naphthalene. Environ Microbiol 15(6):1832-1841. doi:10.1111/1462-2920.12076
Lahme S, Eberlein C, Jarling R, Kube M, Boll M, Wilkes H, Reinhardt R, Rabus R (2012) Anaerobic degradation of 4-methylbenzoate via a specific 4-methylbenzoyl-CoA pathway. Environ Microbiol 14(5):1118-1132. doi:10.1111/j.1462-2920.2011.02693.x
Trautwein K, Grundmann O, Wohlbrand L, Eberlein C, Boll M, Rabus R (2012) Benzoate mediates repression of C(4)-dicarboxylate utilization in "Aromatoleum aromaticum" EbN1. J Bacteriol 194(2):518-528. doi:10.1128/JB.05072-11