Steffi Franke
Contact / Addess:
Phone +49 (0) 341 235 1331
Fax +49 (0) 341 235 450822
Research Group: Organohalide Geomikrobiologie
Helmholtz Centre for Environmental Research - UFZ
Permoserstrasse 15
04318 Leipzig
Curriculum Vitae
| since 09/2014 |
PhD student Helmholtz Centre for Environmental Research – UFZ, Leipzig Department of Isotope Biogeochemistry Hosted by: Friedrich-Schiller-University, Jena Institute for Microbiology, Department for Applied and Ecological Microbiology |
| 10/2013 - 07/2014 |
Research Assistant University of Leipzig Faculty of Chemistry and Minderalogy, Department of Bioanalytical Chemistry in "Ultra Sensitive Protein Detection Unit" |
| 10/2011 - 10/2013 |
M.Sc. in Chemistry University of Leipzig Faculty of Chemistry and Minderalogy, Department of Bioanalytical Chemistry in "Ultra Sensitive Protein Detection Unit" "Entwicklung eines Ligaseassays und dessen Anwendung zur Detektion Alkalischer Phosphatase" Rewarded by GDCh with "Graduation Award in Analytical Chemistry" |
| 10/2008 - 09/2011 |
B.Sc. in Chemistry University of Leipzig Faculty of Chemistry and Mineralogy, Department of Structural Analysis of Biopolymers "Characterisierung von Mutanten der trans-Dienlacton-Hydrolase aus Cupriavidus necator" |
Main Research Interests
- Investigation of reductive dehalogenation using multi-element Compound-Specific Stable Isotope Analysis (CSIA) in the anaerobic microorganisms:
- Dehalococcoides mccartyi strain BTF08 and strain 195
- Thauera chlorobenzoica 3CB-1T
- Proteomics for investigation of reductive dehalogenases involved in "Organohalide Respiration" of chlorinated ethenes and ethanes in Dehalococcoides mccartyi strain BTF08
- Isolation of a pure Dehalococcoides mccartyi from highly enriched culture
- Analytical method development for CSIA, especially for Liquid Chromatography - Isotope Ratio Mass Spectrommetry
Publications
- Franke, S., Kümmel, S., Nijenhuis, I. (2018):
Liquid chromatography/isotope ratio mass spectrometry analysis of halogenated benzoates for characterization of the underlying degradation reaction in Thauera chlorobenzoica CB‐1T
Rapid Commun. Mass Spectrom. 32 (11), 906 - 912 10.1002/rcm.8113 - Franke, S., Lihl, C., Renpenning, J., Elsner, M., Nijenhuis, I. (2017):
Triple-element compound-specific stable isotope analysis of 1,2-dichloroethane for characterization of the underlying dehalogenation reaction in two Dehalococcoides mccartyi strains
FEMS Microbiol. Ecol. 93 (12), fix137 10.1093/femsec/fix137 - Franke, S., Seidel, K., Adrian, L., Nijenhuis, I. (2020):
Dual element (C/Cl) isotope analysis indicates distinct mechanisms of reductive dehalogenation of chlorinated ethenes and dichloroethane in Dehalococcoides mccartyi strain BTF08 with defined reductive dehalogenase inventories
Front. Microbiol. 11 , art. 1507 10.3389/fmicb.2020.01507 - Kruse, S., Türkowsky, D., Birkigt, J., Matturro, B., Franke, S., Jehmlich, N., von Bergen, M., Westermann, M., Rossetti, S., Nijenhuis, I., Adrian, L., Diekert, G., Goris, T. (2021):
Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene
ISME J. 15 (6), 1794 - 1809 10.1038/s41396-020-00887-6 - Lihl, C., Douglas, L.M., Franke, S., Pérez-de-Mora, A., Meyer, A.H., Daubmeier, M., Edwards, E.A., Nijenhuis, I., Sherwood Lollar, B., Elsner, M. (2019):
Mechanistic dichotomy in bacterial trichloroethene dechlorination revealed by carbon and chlorine isotope effects
Environ. Sci. Technol. 53 (8), 4245 - 4254 10.1021/acs.est.8b06643 - Sousa, D.Z., Visser, M., van Gelder, A.H., Boeren, S., Pieterse, M.M., Pinkse, M.W.H., Verhaert, P.D.E.M., Vogt, C., Franke, S., Kümmel, S., Stams, A.J.M. (2018):
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways
Nat. Commun. 9 , art. 239 10.1038/s41467-017-02518-9
Franke, S., Kreisig, T., Buettner, K., Zuchner, T., (2015) One-step assay for the quantification of T4 DNA ligase based on a dark quencher resonance energy transfer. Anal. Bioanal. Chem. 4, 1267-1271. DOI 10.1007/s00216-014-8351-1
Büttner K, Franke S, Kreisig T, Zuechner T. Enzyme Cascade Immunoassay. (2014) EP14150382 (submitted)