Anita O Hidasi
Current position:
PhD at Swiss Federal Institute of Aquatic Science and Technology (Eawag) and Swiss Federal Institute of Technology in Lausanne (EPFL)
Contact:
+41 58 765 5091
Current research
Synthetic glucocorticoids (GCs) are potent endocrine disrupting compounds interfering with the stress response, thus potentially reducing the chance for survival in non-target organisms. GCs have been detected in the aquatic environment in the ng/L range. Until now, a human cell-based reporter gene assay has been proven to be able to detect GC-like activity in environmental water samples.
The main goal of my PhD project is to find GC-action specific and sensitive protein biomarkers with targeted proteomics in order to develop a sensitive bioassay with wild type zebrafish embryos. This bioassay will be used in the EDA of environmental water samples to detect and identify compounds with GC-like activities.
My research is also focusing on the various physiological effects of GC exposure on zebrafish embryos.
Expertise
- Zebrafish (Danio rerio) embryo model
- Proteomics (LC-MS/MS)
- Glucocorticoid effect assessment
- Cell culture
- Molecular biology
- Environmental toxicology
Research interests
- Effect-directed analysis (EDA) of complex environmental contamination
- Risk assessment of contaminants in aquatic ecosystems
- In vivo and in vitro bioassays
- Endocrine disruption in fish models
- Biomarker-based bioassays in ecotoxicology
Curriculum vitae
Since 2012
PhD student at the Department of Environmental Toxicology, Swiss Federal Institute of Aquatic Science and Technology (Eawag) and Swiss Federal Institute of Technology in Lausanne (EPFL). Research topic: “The effects of glucocorticoids in zebrafish embryos”
2010-2012
Master of Science in biochemical engineering at the Budapest University of Technology and Economics. Title of thesis: “Mitochondrial analysis of the effects of herbal plants and BGP-15 during the development of insulin resistance”
2006-2010
Bachelor of Science in biochemical engineering at the Budapest University of Technology and Economics. Title of thesis: “Analysis of epigenetic modifications at the lamin A promoter in cell lines carrying latent Epstein-Barr virus genomes”
Presentations
Anita O Hidasi, Ksenia J Groh, Kristin Schirmer, Marc J-F Suter: Targeted proteomics approach to develop a bioassay detecting environmental glucocorticoids with zebrafish embryos. Poster presentation, 20th IMSC, 24-29 August, 2014, Geneva, Switzerland.
Anita O Hidasi, Ksenia J Groh, Marc J-F Suter, Kristin Schirmer: Novel approaches to assess the effects of glucocorticoids in zebrafish embryos. Oral presentation, SETAC Europe 24th Annual Meeting, 11-15 May, 2014, Basel, Switzerland.
Anita O Hidasi, Ksenia J Groh, Marc J-F Suter, Kristin Schirmer: Targeted proteomics for monitoring glucocorticoid-specific protein biomarkers in zebrafish embryos. Poster presentation, SGMS 2013, 31 October-1 November, 2013, Beatenberg, Switzerland.
Anita O Hidasi, Ksenia J Groh, Marc J-F Suter, Kristin Schirmer: Investigating the effects of glucocorticoids in zebrafish embryos. Poster presentation, 6th Swiss Zebrafish Meeting, 4-5 April 2013, Zürich, Switzerland
Publications
Werner Brack, Selvan Govender, Tobias Schulze, Martin Krauss, Meng Hu, Melis Muz, Juliane Hollender, Kristin Schirmer, Jennifer Schollee, Anita Hidasi, Jaroslav Slobodnik, Zuzana Rabova, Selim Ait-Aissa, Manoj Sonavane, Mario Carere, Marja Lamoree, Pim Leonards, Sara Tufi, Xiyu Ouyang, Merijn Schriks, Kevin Thomas, Ana Catarina de Almeida, Jean Froment, Monika Hammers-Wirtz, Marijan Ahel, Sanja Koprivica, Henner Hollert, Thomas-Benjamin Seiler, Carolina Di Paolo, Andrew Tindall and Petra Spirhanzlova, 2013. EDA-EMERGE: an FP7 initial training network to equip the next generation of young scientists with the skills to address the complexity of environmental contamination with emerging pollutants, Environmental Sciences Europe, 25-18.
