Research Interests
- Single-cell analysis in controlled microenvironments
- Microfluidics
- Single-cell-trapping and cultivation with negative dielectrophoresis (nDEP)
- Quantitative (fluorescence) microscopy
since 2014
Scientist, Department of Solar Materials
Helmholtz Center for Environmental Research - UFZ, Leipzig
2010 - 2014
Dissertation at the Laboratory of Chemical Biotechnology,
TU Dortmund University, Germany, under the supervision of Prof. Dr. Andreas Schmid
2010 - 2012
Research assistant, Leibniz-Institut für analytische Wissenschaften ISAS – e.V., Dortmund, Germany
2009 - 2010
Project engineer, DSM Anti-Infectives, Delft, Netherlands
2002 - 2009
Studies of Biochemical Engineering, TU Dortmund University, Germany, Degree: Diplom-Ingenieur (TU)
Index:
You could use our publication index for further requests.
2024 (1)
- Grünberger, A., Bahnemann, J., Dusny, C. (2024):
Editorial overview: Analytical Biotechnology: It's all about getting smaller
Curr. Opin. Biotechnol. 85 , art. 103029 10.1016/j.copbio.2023.103029
2023 (4)
- Garmasukis, R., Hackl, C., Dusny, C., Elsner, C., Charvat, A., Schmid, A., Abel, B. (2023):
Cryo-printed microfluidics enable rapid prototyping for optical-cell analysis
Microfluid. Nanofluid. 27 , art. 5 10.1007/s10404-022-02613-4 - Schirmer, M., Dusny, C. (2023):
Microbial single-cell mass spectrometry: status, challenges, and prospects
Curr. Opin. Biotechnol. 83 , art. 102977 10.1016/j.copbio.2023.102977 - Smaluch, K., Dusny, C., Kohlheyer, D., Grünberger, A. (2023):
Mikroskalige Massenbilanzierung in mikrofluidischen Umgebungen
Biospektrum 29 (5), 534 - 535 10.1007/s12268-023-1978-8 - Smaluch, K., Wollenhaupt, B., Steinhoff, H., Kohlheyer, D., Grünberger, A., Dusny, C. (2023):
Assessing the growth kinetics and stoichiometry of Escherichia coli at the single-cell level
Eng. Life Sci. 23 (1), e2100157 10.1002/elsc.202100157
2022 (6)
- Blaha, M.E., Hasan, S., Dusny, C., Belder, D. (2022):
Fluorescence lifetime activated droplet sorting (FLADS) for label-free sorting of Synechocystis sp. PCC6803
Lab Chip 22 (8), 1604 - 1614 10.1039/d2lc00032f - Dusny, C. (2022):
Microfluidic single-cell analytics
In: Bahnemann, J., Grünberger, A. (eds.)
Microfluidics in biotechnology
Adv. Biochem. Eng. Biotechnol. 179
Springer Nature, p. 159 - 189 10.1007/10_2020_134 - Martinez, J.A., Delvenne, M., Henrion, L., Moreno, F., Telek, S., Dusny, C., Delvigne, F. (2022):
Controlling microbial co-culture based on substrate pulsing can lead to stability through differential fitness advantages
PLoS Comput. Biol. 18 (10), e1010674 10.1371/journal.pcbi.1010674 - Wink, K., van der Loh, M., Hartner, N., Polack, M., Dusny, C., Schmid, A., Belder, D. (2022):
Quantification of biocatalytic transformations by single microbial cells enabled by tailored integration of droplet microfluidics and mass spectrometry
Angew. Chem.-Int. Edit. 61 (29), e202204098 10.1002/anie.202204098 - Xiong, B.-J., Kleinsteuber, S., Sträuber, H., Dusny, C., Harms, H., Wick, L.Y. (2022):
Impact of fungal hyphae on growth and dispersal of obligate anaerobic bacteria in aerated habitats
mBio 13 (3), e00769-22 10.1128/mbio.00769-22 - Xiong, B.-J., Stanley, C.E., Dusny, C., Schlosser, D., Harms, H., Wick, L.Y. (2022):
pH distribution along growing fungal hyphae at microscale
J. Fungi 8 (6), art. 599 10.3390/jof8060599
2021 (5)
- Bühler, K., Bühler, B., Klähn, S., Krömer, J.O., Dusny, C., Schmid, A. (2021):
Biocatalytic production of white hydrogen from water using cyanobacteria
In: Rögner, M. (ed.)
Photosynthesis: Biotechnological applications with microalgae
De Gruyter, Berlin ; Boston, p. 279 - 306 10.1515/9783110716979-011 - Bühler, K., Krömer, J.O., Klähn, S., Bühler, B., Dusny, C., Schmid, A. (2021):
Weißer Wasserstoff made in Leipzig
Biospektrum 27 (3), 335 10.1007/s12268-021-1572-x - Dusny, C., Schmid, A. (2021):
The metabolic flux probe (MFP)—Secreted protein as a non-disruptive information carrier for 13C-based metabolic flux analysis
Int. J. Mol. Sci. 22 (17), art. 9438 10.3390/ijms22179438 - Krafft, B., Tycova, A., Urban, R.D., Dusny, C., Belder, D. (2021):
Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water
Electrophoresis 42 (1-2), 86 - 94 10.1002/elps.202000048 - Xiong, B.-J., Dusny, C., Wang, L., Appel, J., Lindstaedt, K., Schlosser, D., Harms, H., Wick, L. (2021):
Illuminate the hidden: in vivo mapping of microscale pH in the mycosphere using a novel whole-cell biosensor
ISME Commun. 1 , art. 75 10.1038/s43705-021-00075-3
2020 (3)
- Dusny, C., Grünberger, A. (2020):
Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding
Curr. Opin. Biotechnol. 63 , 26 - 33 10.1016/j.copbio.2019.11.001 - Lohse, M., Dusny, C., Kaesler, J., Lechtenfeld, O.J. (2020):
MRMS powered single cell metabolomics – Quantification of picogram amounts of a biocatalytic product from few living cells
Bruker Daltonics, 10.5281/zenodo.3947765 - Schirmer, M., Wink, K., Ohla, S., Belder, D., Schmid, A., Dusny, C. (2020):
Conversion efficiencies of a few living microbial cells detected at a high throughput by droplet-based ESI-MS
Anal. Chem. 92 (15), 10700 - 10708 10.1021/acs.analchem.0c01839
2019 (1)
- Dusny, C., Lohse, M., Reemtsma, T., Schmid, A., Lechtenfeld, O.J. (2019):
Quantifying a biocatalytic product from a few living microbial cells using microfluidic cultivation coupled to FT-ICR-MS
Anal. Chem. 91 (11), 7012 - 7018 10.1021/acs.analchem.9b00978
2018 (1)
- Oehling, V., Klaassen, P., Frick, O., Dusny, C., Schmid, A. (2018):
L-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain
Biotechnol. Biofuels 11 , art. 231 10.1186/s13068-018-1231-8
2017 (3)
- Delvigne, F., Baert, J., Sassi, H., Fickers, P., Grünberger, A., Dusny, C. (2017):
Taking control over microbial populations: Current approaches for exploiting biological noise in bioprocesses
Biotechnol. J. 12 (7), art. 1600549 10.1002/biot.201600549 - Fritzsch, F.S.O., Blank, L.M., Dusny, C., Schmid, A. (2017):
Miniaturized octupole cytometry for cell type independent trapping and analysis
Microfluid. Nanofluid. 21 (8), art. 130 10.1007/s10404-017-1969-5 - Rosenthal, K., Oehling, V., Dusny, C., Schmid, A. (2017):
Beyond the bulk: disclosing the life of single microbial cells
FEMS Microbiol. Rev. 41 (6), 751 - 780 10.1093/femsre/fux044
2016 (2)
- Dusny, C., Schmid, A. (2016):
The MOX promoter in Hansenula polymorpha is ultrasensitive to glucose-mediated carbon catabolite repression
FEMS Yeast Res. 16 (6), fow067 10.1093/femsyr/fow067 - Dusny, C., Schmid, A. (2016):
Neue Einblicke in die Welt isolierter Mikroben
Biospektrum 22 (7), 708 - 711 10.1007/s12268-016-0748-2
2015 (4)
- Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., Schmid, A. (2015):
Technical bias of microcultivation environments on single-cell physiology
Lab Chip 15 (8), 1822 - 1834 10.1039/c4lc01270d - Dusny, C., Schmid, A. (2015):
Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes
Environ. Microbiol. 17 (6), 1839 - 1856 10.1111/1462-2920.12667 - Dusny, C., Schmid, A. (2015):
Challenging biological limits with microfluidic single cell analysis
Microb. Biotechnol. 8 (1), 23 - 25 10.1111/1751-7915.12252 - Rosenthal, K., Falke, F., Frick, O., Dusny, C., Schmid, A. (2015):
An inert continuous microreactor for the isolation and analysis of a single microbial cell
Micromachines 6 (12), 1836 - 1855 10.3390/mi6121459
2013
- Fritzsch F. S. O., Rosenthal K., Kampert A., Howitz S., Dusny C., Blank L. M. and Schmid A. (2013)
Picoliter nDEP traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments.
Lab on a Chip, 13(3):397-408, DOI-Link
2012
- Dusny C., Fritzsch F. S. O., Frick O. and Schmid A. (2012)
Isolated Microbial Single Cells and Resulting Micropopulations Grow Faster in Controlled Environments.
Applied and Environmental Microbiology, 78(19):7132-7136, DOI-Link - Fritzsch F. S. O.*, Dusny C.*, Frick O. and Schmid A. (2012)
Single Cell Analysis in Biotechnology, Systems Biology, and Biocatalysis.
Annual Review of Chemical and Biomolecular Engineering, 3:129-155, DOI-Link
* These authors contributed equally to this work.
2009
- Ruinatscha R., Dusny C., Bühler K. and Schmid A. (2009)
Productive asymmetric styrene epoxidation based on a next generation electroenzymatic methodology.
Advanced Synthesis and Catalysis, 351:2505-2515, DOI-Link
2018
- Dusny, C., Schirmer, M. and Schmid, A. (2018)
Dissecting biocatalytic heterogeneity: Measuring productivities of microbial populations, one cell at a time.
Himmelfahrtstagung 2018: Heterogeneities - A key for understanding and upscaling of bioprocesses in up- and downstream processing, 7th - 9th May 2018, Magdeburg Germany
2017
- Dusny C. and Schmid A. (2017)
Microfluidic compartimentalization for whole-cell biocatalysis.
KompaTech Innovationsforum: Workshop „Kompartimentierte Biotechnologie,
23rd - 24th January 2016, Weimar, Germany
2016
- Dusny C. (2016)
Microbial Photocatalysis:
Microbes in service for green energy and materials.
HighGrade Module ‘Microbial Services in Times of Global Change,
11th March 2016, UFZ, Leipzig, Germany - Dusny C. (2016)
Isolation und Analyse von mikrobiellen Zellen unter kontrollierten Kultivierungsbedingungen.
Seminar „Mikrofluidik und Einzelzell-Analyse in der Biotechnologie“,
30th June 2016, RWTH Aachen, Germany - Dusny C. and Schmid A. (2016)
Quantitative physiology of single microbial cells in controlled extracellular environments.
SoMA meets IZI, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany
1st September 2016 - Dusny C. and Schmid A. (2016)
The Envirostat- a Microfluidic Single Cell Analysis Platform For Non-contact Cultivation And Analysis of Single Microorganisms.
bionection - Technology transfer in life sciences,
18th - 19th October 2016, Halle, Germany - Dusny C. and Schmid A. (2016)
Single cell microfluidics for whole-cell biocatalysis.
Workshop der DFG-Forschergruppe 2177: Integrierte chemische Mikrolaboratorien,
20th - 21st November 2016, Lutherstadt Wittenberg, Germany
2015
- Dusny C. and Schmid A. (2015)
Biotechnology of single cells in controlled environments.
The 3rd International Conference IMPLEMENTATION OF MICROREACTOR TECHNOLOGY IN BIOTECHNOLOGY,
10th - 13th May 2015, Opatija, Croatia - Dusny C. (2015)
Der Envirostat: kontaktlose und markierungsfreie Sortierung, Isolation und Analyse von Zellen und Biopartikeln unter kontrollierten Kultivierungsbedingungen.
Seminar „Mikrofluidik und Einzelzell-Analyse in der Biotechnologie“,
25th June 2015, RWTH Aachen, Germany - Dusny C. and Schmid A. (2015)
Microfluidics enable quantitative physiology of single microbial cells in controlled extracellular environments.
6th International Conference On Analysis Of Microbial Cells at the Single Cell Level,
19th - 22nd July 2015, Retz, Austria
2014
- Dusny C., Grünberger A., Wiechert W. and Schmid A. (2014)
From extracellular environment to functional phenotype: comparison of single cell physiology in static and steady environments.
3rd BioProScale Symposium,
02nd - 04th April 2014, Berlin, Germany - Dusny C., Grünberger A., Wiechert W., Kohlheyer D. and Schmid A. (2014)
Quantitative physiology of single cells for linking phenotype and environment (invited Keynote talk and Session chair).
23rd Annual Conference of the German Society for Cytometry,
15th - 17th October 2014, Dresden, Germany
2013
- Dusny C., Rosenthal K. and Schmid A. (2013)
Microbial single cell analysis under controlled cultivation conditions.
Microfluidics for Systems Biology and Bioprocess Development 2013,
21st March 2013, Frankfurt am Main, Germany - Dusny C., Fritzsch F. S. O., Rosenthal K., Oehling V., Frick O. and Schmid A. (2013)
The Envirostat: physiology of isolated single cells in controlled microenvironments.
Biocatalysis Workshop „Brain meets Lab of Chemical Biotechnology“,
12th July 2013, Brain AG, Zwingenberg, Germany
2012
- Dusny C., Fritzsch F. S. O., Rosenthal K., Frick O. and Schmid A.(2012)
The Envirostat: A picoliter bioreactor for the quantitative analysis of isolated single cells in controlled microenvironments.
Micro Flow Chemistry And Biology Workshop -1,
7th – 8th November 2012, Dead Sea, Jordan
2014
- Dusny C., Lindstaedt K. and
Schmid A. (2014)
Let there be light – quantitative analysis of phototrophic cyanobacteria in microfluidic environments.
Bioproscale Symposium,
6. – 8. April 2016, Berlin, Germany - Rosenthal K., Dusny C., Oehling V., Frick O. and Schmid A. (2014)
Analysis of a single-cell: Imprisoned, but feeling well.
5th annual CLIB-graduate cluster retreat,
12. – 14. February 2014, Lünen, Germany - Dusny C., Fritzsch F. S. O., Rosenthal K. and Schmid A. (2014)
Quantitative single cell analysis reveals faster growth of isolated microbes in controlled microenvironments in comparison to populations
(Best poster award).
SysBio2014 Advanced Lecture Course on Systems Biology,
02. – 08. March 2014, Innsbruck, Austria - Rosenthal K., Dusny C., Oehling V., Frick O. and Schmid A. (2014)
The Envirostat 3.0: Microfluidic chip development for sampling of metabolites secreted by a single cell.
3rd BioProScale Symposium,
02. - 04. March 2014, Berlin, Germany
2013
- Rosenthal K., Fritzsch F. S. O., Dusny C., Oehling V., Frick O. and
Schmid A. (2013)
The Way for Single Cell Analysis: Pick the right, hold it tight, - then do a sight, if it’s bright.
4th annual CLIB-graduate cluster retreat,
13. – 15. February 2013, Lünen, Germany - Oehling V., Fritzsch F. S. O., Dusny C., Rosenthal K., Kortmann H.,
Frick O. and Schmid A. (2013)
Single cell analysis of ethanol producing Saccharomyces cerevisiae.
3rd BE-Basic Symposium,
5. - 7. February 2013, Noordwijkerhout, The Netherlands
2012
- Dusny C., Fritzsch F. S. O., Rosenthal K., Frick O. and Schmid A. (2012)
Quantitative physiology with isolated single cells and micropopulations in controlled microenvironments employing a picoliter bioreactor.
The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS),
28. October – 1. November 2012, Okinawa, Japan - Rosenthal K., Fritzsch F. S. O., Dusny C., Oehling V.,
Frick O. and Schmid A. (2012)
Fluoreszenz gestützte Analytik von Mikroorganismen im mikrofluidischen Einzelzellreaktor.
ProcessNet-Jahrestagung,
10. - 13. September 2012, Karlsruhe, Germany - Rosenthal K., Fritzsch F. S. O., Dusny C., Frick O.,
Blank L. M. and Schmid A. (2012)
An Overview: Microbioreactors and Analytics for Systems biology on Single Cell Level .
2nd BioProScale Symposium,
14. - 16. March 2012, Berlin, Germany - Fritzsch F. S. O., Oehling V., Rosenthal K., Dusny C., Kampert A.,
Kortmann H., Frick O., Blank L. M. and Schmid A. (2012)
Contactless single cell analysis of industrial relevant bacteria in controlled microenvironments.
2nd BioProScale Symposium,
14. - 16. March 2012, Berlin, Germany - Dusny C., Fritzsch F. S. O., Rosenthal K., Parolina G., Frick O. and Schmid A. (2012)
Beyond the bulk: Analysis and cultivation of single yeast and bacteria in precisely controlled extracellular environments (Best poster award).
2nd BioProScale Symposium,
14. - 16. March 2012, Berlin, Germany
2011
- Rosenthal K., Fritzsch F. S. O., Dusny C., Frick O., Blank L. M.
and Schmid A. (2011)
Single Cell Analysis in Biotechnology A: An Overview: From defined Microenvironments towards Systems Biology.
Bubble Tech to Bio App – LAB-ON-A-CHIP,
17. - 18. October 2011, Saarbruecken, Germany - Fritzsch F. S. O., Rosenthal K., Dusny C., Kampert A.,
Kortmann H., Frick O., Blank L. M. and Schmid A. (2011)
Single Cell Analysis in Biotechnology B: The Envirostat 2.0 – A next generation microfluidic platform for single cell analysis in controlled microenvironments.
Bubble Tech to Bio App – LAB-ON-A-CHIP,
17. - 18. October 2011, Saarbruecken, Germany - Dusny C., Fritzsch F. S. O., Rosenthal K., Parolina G.,
Oehling V., Frick O., Blank L. M. and Schmid A. (2011)
Single Cell Analysis in Biotechnology C: Time-resolved quantitative physiology of trapped single yeast and bacteria in defined microenvironments.
Bubble Tech to Bio App – LAB-ON-A-CHIP,
17. - 18. October 2011, Saarbruecken, Germany - Dusny C., Fritzsch F. S. O., Kortmann H.,
Blank L. M. and Schmid A. (2011)
Towards quantitative physiology with single yeast.
bio.dortmund,
28. September 2011, Dortmund, Germany - Fritzsch F. S. O., Dusny C., Kortmann H.,
Blank L. M. and Schmid A. (2011)
Single cell analysis.
Nano! Nutzen und Visionen einer neuen Technology, DASA,
15. July 2011, Dortmund, Germany - Dusny C., Fritzsch F. S. O., Blank L. M. and Schmid A. (2011)
Towards quantitative physiology with single yeast
(Best poster award).
Leibniz-Institute for Analytical Sciences – ISAS - e. V. Summer School 2011,
07. July 2011, Dortmund, Germany
2010
Fritzsch F. S. O., Dusny C., Kortmann H.,
Blank L. M. and Schmid A. (2010)
Single Cell Analysis.
ISAS - Evaluation,
30. September 2010, Dortmund, Germany