Dr. Claus Härtig

Contact

Dr. Claus Härtig
Scientist

Department of Environmental Microbiology
Working Group Microbial Bioelectrocatalysis and Bioelectrotechnology
Helmholtz Centre for
Environmental Research - UFZ
Permoserstr. 15, 04318 Leipzig, Germany

Phone +49 341 235-1326
claus.haertig@ufz.de

Claus Härtig

CV / Scientific Career

1976-1981

Study of Chemistry at the Technical University of Freiberg,
Stipend Award 1978-1981

1981

Diploma in Chemistry, Technical University of Freiberg

1981-1984

Research Fellowship at the Technical University of Freiberg

1984

PhD, Technical University of Freiberg,
‘Forming and Isomorphism of Solid Solutions in the Sytem Jarosite-Alunite’

1984-1986

Scientist at the Academy of Sciences of the GDR, Institute of Biotechnology Leipzig

1986-1988

Postgraduate Studies in Biotechnology and Genetic Engineering at the University of Jena and the Academy of Sciences of the GDR, Central Institute of Microbiology and Experimental Therapy Jena

1988-1992

Senior Scientist at the Academy of Sciences of the GDR, Institute of Biotechnology Leipzig

Research fields:

Renewable raw materials
Bioconversion of lignin
White-rot fungi

since 1992

Senior Scientist at the Helmholtz Centre for Environmental Research-UFZ Leipzig-Halle, Department of Environmental Microbiology

Research fields:

Bacterial stress physiology
Microbial community analysis
Metabolomics and databases


Research interests

Identification of microorganisms from environmental samples via comparison of fatty acid patterns determined by gas chromatography and the use of commercial (SHERLOCK/MIDI) and self-generated databases

Development of lipid extraction and fractionation protocols for samples of biological origin (cells, tissues, body fluids). Optimization of methods with respect to the hydrophobic properties of analyte, matrix and solvents used for extraction

Reliable fatty acid analyses with focus on determination of double bond position and cis/trans isomers. Compiling of a multidimensional gas chromatography/mass spectrometry fatty acid methyl ester database and its use for a doubtless identification of fatty acids (FAME_ID® = retention time locked database for more than 180 compounds, includes mass spectra and retention indices on several columns of different polarity)

Processing of data from gas chromatography/mass spectrometry in metabolomics experiments. It includes data acquisition from plants and microbial samples followed by the application of alignment tools and multivariate techniques of data analysis (cluster analysis, principal component analysis)

Use of different cultivation techniques for bacteria (batch, chemostat, auxostat) to realize different growth conditions and in connection with them limited or inhibited growth. Reproducible generation of cell material at different physiological states

Stress physiology and adaptation of microbial lipid membranes as a response to environmental changes (temperature, toxic chemicals, and nutrients). Measurement of changes in membrane fluidity by temperature dependent fluorescence anisotropy spectroscopy and infrared spectroscopy. Quantitative analyses of fatty acid alterations in bacteria to get insight in their stability and resiliency and to understand the ecophysiological importance of adaptation

Use of phospholipid fatty acids as biomarker for the description of microbial biodiversity in complex ecosystems. Structural and functional community analyses and impacts of toxicants on their dynamics. Estimation of microbial biomass in such heterogeneous samples


Co-operations / Projects

  • SAFIRA II Research Program - Compartment Transfer (CoTra)

Teaching

Lectures, courses and practical trainings in Physical Chemistry, Basic Microbiology and Ecological Biochemistry at Technical University of Freiberg and at University of Leipzig


Publications